Mechanism of enzymic acetylation of des-acetyl citrate lyase.
نویسندگان
چکیده
A new enzyme, acetate:SH-[acyl-carrier-protein] enzyme ligase (AMP), has been purified about 30-fold from cell-free extracts of Klebsiella aerogenes. The enzyme, in the presence of acetate and ATP, catalyzes the acetylation of enzymically inactive des-acetyl citrate lyase to enzymically active citrate lyase (EC 4.1.3.6). Acetate and ATP could be replaced by acetyl adenylate. Acetyl-CoA can act as acetyl donor in this activation only if trace amounts of adenine nucleotides and auxiliary enzymes are present. These allow formation of acetyl adenylate in acetate- and ATP-generating reactions.
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ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 71 5 شماره
صفحات -
تاریخ انتشار 1974